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Journal: Journal of Translational Medicine
Article Title: Hsa_circ_0016662 acts as a prognostic biomarker and promotes the progression of hepatocellular carcinoma via autophagy regulation
doi: 10.1186/s12967-025-06763-1
Figure Lengend Snippet: Validation of hsa_circ_0016662 regulatory network in real-world cohort and HCC cell lines. (A) - (B) The expression of hsa_circ_0016662 and hsa-miR-22-3p validated in Guangxi local HCC samples by qRT-PCR ( n = 31). (C) - (D) Representative IHC staining images of YWHAZ in local HCC tissues and adjacent normal liver tissues. (E) The IHC scores of YWHAZ were compared between local HCC tissues and adjacent normal liver tissues ( n = 36). (F) - (H) qRT-PCR revealed the mRNA expression of hsa_circ_0016662, hsa-miR-22-3p and YWHAZ in HCC cell lines comparing to the normal liver cell L02. (I) The protein expression of YWHAZ in HCC cell lines and L02 detected by western blot. * : P < 0.05, *** : P < 0.001
Article Snippet: Six
Techniques: Biomarker Discovery, Expressing, Quantitative RT-PCR, Immunohistochemistry, Western Blot
Journal: Journal of Translational Medicine
Article Title: Hsa_circ_0016662 acts as a prognostic biomarker and promotes the progression of hepatocellular carcinoma via autophagy regulation
doi: 10.1186/s12967-025-06763-1
Figure Lengend Snippet: Knockdown of hsa_circ_0016662 suppressed HCC progression in vivo. (A) Subcutaneous xenografts of Huh7 cells dissected from nude mice. (B) - (C) The tumor volume and tumor weight were calculated and compared in samples from the subcutaneous xenograft model. (D) Hsa_circ_0016662 expression in the subcutaneous xenograft model was detected by qRT-PCR. (E) Representative staining images of HE and Ki-67 in tumors from the subcutaneous xenograft model. (F) Representative HE images of lung metastatic foci derived from the mice of metastatic model. (G) The metastatic proportion was calculated in the lung metastatic model. ** P < 0.001, *** : P < 0.001, **** : P < 0.0001
Article Snippet: Six
Techniques: Knockdown, In Vivo, Expressing, Quantitative RT-PCR, Staining, Derivative Assay